Bone Abstracts

Osteoclasts are the major bone resorbing cells, essential for bone turnover and development. Human osteoclasts can be generated in vitro by stimulation of peripheral blood mononuclear cells (PBMCs) with M-CSF and RANK-L. Seeding fully differentiated osteoclasts onto mineralized surfaces facilitates the analysis of molecular interactions between the osteoclast and the mineralized matrix.Currently, a widely used protocol for harvesting osteoclasts...